Gt(ROSA)26Sor^{tm2.1(mix1b-mCherry)Mgn}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(mix1b-mCherry)Mgn}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Mark A Magnuson
• MGI ID: MGI:5476468
• Synonyms: -228/AR8-TA-mCherry, Rosa26^{(228.AR8.TA.mCherry)Hri}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(mix1b-mCherry)Mgn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:195112
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: This allele was created by RMCE in Gt(ROSA)26Sor^tm1Mgn. The original selections cassettes (puromycin N-acetyltransferase and a truncated form of HSV1 thymidine kinase and EM7-driven neomycin resistance) were replaced by a cassette composed of ROSA26 upstream sequence deleted from the start codon to -228, 8 copies of the 50-bp activin response element (ARE) from the Xenopus laevis Mix1b promoter controlling expression of the mCherry reporter. An upstream FLP flanked hygromycin selection cassette was subsequently removed by flp recombinase. (J:195112)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

Notes

This allele was generate from Gt(ROSA)26Sor^tm1Mgn.

References

• Original: J:195112 Serup P, et al., Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. Dis Model Mech. 2012 Nov;5(6):956-66
• All: 1 reference(s)