Tg(Itgax-BCL2)1Ple
Transgene Detail

Summary

• Symbol: Tg(Itgax-BCL2)1Ple
• Name: transgene insertion 1, Philippe Lesnik
• MGI ID: MGI:5475551
• Synonyms: DC-hBcl-2, Tg CD11c-hBcl2
• Transgene: Tg(Itgax-BCL2)1Ple Location: unknown
• Alliance: Tg(Itgax-BCL2)1Ple page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Itgax-BCL2)1Ple expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  BCL2 (596)

  Bcl2	(MGI:88138)

• Mutation details: The transgene contains the mouse dendritic cell-specific integrin alpha X (also called Cd11c) promoter, from nucleotide -821 to the ATG translation initiation codon, followed by a cDNA encoding the human B cell leukemia/lymphoma 2 protein. Real-time quantitative PCR analysis demonstrated expression of human BCL2 mRNA in spleen, lung and thymus, which contain dendritic cells, but not in heart or skeletal muscle of transgenic mice. Expression of human BCL2 protein in bone marrow-derived dendritic cells (BMDCs) was shown by intracellular staining and flow cytometry. Restriction of the protein's expression to dendritic cells was verified by double staining of splenocytes for human BCL2 and mouse T cell, B cell, macrophage and dendritic cell specific markers; confocal microscopy further showed correct localization of the protein to the mitochodria and endoplasmic reticulum. (J:134867)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:134867 Gautier EL, et al., Enhanced dendritic cell survival attenuates lipopolysaccharide-induced immunosuppression and increases resistance to lethal endotoxic shock. J Immunol. 2008 May 15;180(10):6941-6
• All: 5 reference(s)