Tg(Mt1-SERPINE1)1Upj
Transgene Detail

Summary

• Symbol: Tg(Mt1-SERPINE1)1Upj
• Name: transgene insertion 1, The Upjohn Company
• MGI ID: MGI:5475344
• Synonyms: PAI-1
• Transgene: Tg(Mt1-SERPINE1)1Upj Location: unknown
• Alliance: Tg(Mt1-SERPINE1)1Upj page

Transgene origin

• Strain of Origin: (C57BL/6J x SJL/J)F1/J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Mt1-SERPINE1)1Upj expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SERPINE1 (5054)

  Serpine1

  (MGI:97608)

• Mutation details: This transgene comprises an 1,800 bp DNA fragment containing the mouse metallothionein 1 promoter followed by a 1,397 bp cDNA encoding human SERPINE1 (serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1; also called endothelial cell plasminogen activator inhibitor 1) and the bovine growth homone polyadenylation signal. Whereas SERPINE1 mRNA is elevated in livers of 4 day old transgenic mice, by 28 days it has dropped to control levels. Four day-old transgenic mice have greatly elevated levels of the protein, as measured by enzyme-linked immunosorbent assay (ELISA) using a monoclonal anti-SERPINE1 antibody, in spleen, liver and kidney, and lower, but still markedy elevated levels in heart, brain and lung. The liver protein level has diminished >50% by 7 days and 10-fold by 28 days, but remains ~3.5-fold that of controls. Injection of 28 day old mice with zinc sulfate (ZnSO4) induces expression of the protein in plasma (20-fold increase) and in all tissues analyzed. (J:194516)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:194516 Erickson LA, et al., Development of venous occlusions in mice transgenic for the plasminogen activator inhibitor-1 gene. Nature. 1990 Jul 5;346(6279):74-6
• All: 1 reference(s)