About   Help   FAQ
Chattm2(cre)Lowl
Targeted Allele Detail
Nomenclature
Symbol: Chattm2(cre)Lowl
Name: choline acetyltransferase; targeted mutation 2, Bradford B Lowell
MGI ID: MGI:5475195
Synonyms: ChAT-IRES-Cre
Gene: Chat  Location: Chr14:32408203-32465909 bp, - strand  Genetic Position: Chr14, 19.4 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:169562
Parent Cell Line:  W4 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Mutation
description
Allele Type:    Targeted (Recombinase)
Mutation:    Insertion
 
Mutation detailsAn optimized internal ribosome entry sequence (IRES) fused to cre recombinase followed by a neomycin resistance cassette flanked FRT sites was inserted after the ChAT stop codon in BAC (bMQ185k21) using homologous recombination in bacteria. A genomic ChAT fragment with 4kb homology arms on either side of the IRES-Cre insertion site was lifted out from the BAC using reverse recombineering techniques and transformed into ES cells. This allele was used to generate Chattm1(cre)Lowl. (J:169562)
Recombinase
activity
Activity:
Loading...
Driver: Chat     Summary of all recombinase alleles driven by Chat. Comparative matrix view of recombinase activities
 

Phenotypes
Loading...
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Loading...
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Chat Mutation:  13 strains or lines available
References
Original:  J:169562 Rossi J, et al., Melanocortin-4 receptors expressed by cholinergic neurons regulate energy balance and glucose homeostasis. Cell Metab. 2011 Feb 2;13(2):195-204
All:  129 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
12/03/2019
MGI 6.14
The Jackson Laboratory