Cers1tm1.1Kwi
Targeted Allele Detail
|
Symbol: |
Cers1tm1.1Kwi |
Name: |
ceramide synthase 1; targeted mutation 1.1, Klaus Willecke |
MGI ID: |
MGI:5475110 |
Gene: |
Cers1 Location: Chr8:70768425-70784238 bp, + strand Genetic Position: Chr8, 34.15 cM
|
Alliance: |
Cers1tm1.1Kwi page
|
|
Foliation defects and cerebellar neurodegeneration in Cers1tm1.1Kwi/Cers1tm1.1Kwi mice
Show the 1 phenotype image(s) involving this allele.
|
|
|
|
Allele Type: |
|
Targeted (Null/knockout) |
Mutation: |
|
Insertion
|
|
|
Mutation details: Exon 1 was replaced with a modified exon 1 lacking the translation initation site. An FRT-flanked neo cassette was inserted upstream of exon 2. An EGFP gene with a splice acceptor and splice donor replaced part of exon 3 with additional translation reinitiation sites. Flp-mediated recombination removed the neo cassette. Western blot analysis confirmed the absence of protein expression in the forebrain, cerebellum, spinal cord and muscle and presence of Gdf1 protein expression in E10 embryos. No GFP expression is detected by western blot analysis.
(J:193419)
|
|
Generation of the Cers1tm1.1Kwi allele |
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
Original: |
J:193419 Ginkel C, et al., Ablation of neuronal ceramide synthase 1 in mice decreases ganglioside levels and expression of myelin-associated glycoprotein in oligodendrocytes. J Biol Chem. 2012 Dec 7;287(50):41888-902 |
All: |
1 reference(s) |
|