Pikfyve^tm1Tssk
Targeted Allele Detail

Summary

• Symbol: Pikfyve^tm1Tssk
• Name: phosphoinositide kinase, FYVE type zinc finger containing; targeted mutation 1, Takehiko Sasaki
• MGI ID: MGI:5474980
• Synonyms: PipkIII^neo
• Gene: Pikfyve Location: Chr1:65225842-65317854 bp, + strand Genetic Position: Chr1, 32.94 cM, cytoband C3
• Alliance: Pikfyve^tm1Tssk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193689
• Parent Cell Line: E14K (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 were replaced with a lacZ-pgk-neo cassette via homologous recombination. Immunoblot and RT-PCR confrimed the absence of protein and RNA expression in homozygous ES cells. (J:193689)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pikfyve Mutation: 114 strains or lines available

References

• Original: J:193689 Takasuga S, et al., Critical roles of type III phosphatidylinositol phosphate kinase in murine embryonic visceral endoderm and adult intestine. Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1726-31
• All: 1 reference(s)