Igs1^{tm8(CAG-Bgeo,-HIST1H2BB/EGFP)Nat}
Targeted Allele Detail

Summary

• Symbol: Igs1^{tm8(CAG-Bgeo,-HIST1H2BB/EGFP)Nat}
• Name: intergenic site 1; targeted mutation 8, Jeremy Nathans
• MGI ID: MGI:5474845
• Synonyms: Iis1^{tm8(CAG-Bgeo,-HIST1H2BB/EGFP)Nat}, Z/H2B-GFP
• Gene: Igs1 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Igs1^{tm8(CAG-Bgeo,-HIST1H2BB/EGFP)Nat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:193332
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: A site 5' of the Ubb gene was targeted with a construct composed of a CMV/beta-actin enhancer and promoter (CAG), a floxed beta-geo with three transcription termination sites and an HIST1H2BB and GFP (H2B-GFP) fusion. Cre-mediated excision of the floxed beta-geo construct is required to achieve expression of the H2B-GFP cassette. (J:193332)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igs1 Mutation: 10 strains or lines available

References

• Original: J:193332 Wang Y, et al., Norrin/Frizzled4 signaling in retinal vascular development and blood brain barrier plasticity. Cell. 2012 Dec 7;151(6):1332-44
• All: 2 reference(s)