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Transgene Detail
Symbol: Tg(Mchr1-cre)1Emf
Name: transgene insertion 1, Eleftheria Maratos-Flier
MGI ID: MGI:5474277
Synonyms: Mchr1-cre BAC tg
Transgene: Tg(Mchr1-cre)1Emf  Location: unknown  
Strain of Origin:  C57BL/6J
Transgene Type:    Transgenic (Recombinase)
Mutation:    Insertion
Mutation detailsA cre recombinase sequence (1032 bp) was inserted downstream of the Mchr1 promoter on the RP23-202N16 BAC via homologous recombination/BAC recombineering; this replaced nucleotides 4-41 from exon 1 and was designed to disable transcription/translation of the Mchr1 protein from the BAC Mchr1 locus. An frt-flanked kanamycin selection cassette used during homologous recombination/BAC recombineering was removed by transient FLP plasmid injection. The resulting ~198 kbp Mchr1-cre BAC transgene was injected into C57BL/6J embryos to establish transgenic founder animals. Founder animals were bred to C57BL/6J mice to generate independent founder lines. One founder line was chosen. (J:194588)
Schematic of the Tg(Mchr1-cre)1Emf transgene
 Tissue activity of this recombinase allele
Driver: Mchr1     Summary of all recombinase alleles driven by Mchr1. Comparative matrix view of recombinase activities

View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:194588 Chee MJ, et al., Neurochemical charaterization of neurons expressing melanin-concentrating hormone receptor 1 in the mouse hypothalamus. J Comp Neurol. 2013 Jul;521(10):2208-34
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
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