Actl10^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Actl10^{tm1a(EUCOMM)Wtsi}
• Name: actin-like 10; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:5473172
• Gene: Actl10 Location: Chr2:154393696-154395196 bp, + strand Genetic Position: Chr2, 76.79 cM
• Alliance: Actl10^{tm1a(EUCOMM)Wtsi} page
• IMPC: Actl10 gene page

Mutation origin

• Mutant Cell Lines: EPD0920_2_A10, EPD0920_2_A12, EPD0920_2_D09, EPD0920_2_D10, EPD0920_2_D12, EPD0920_2_E09, EPD0920_2_E11, EPD0920_2_E12, EPD0920_2_F09, EPD0920_2_H09, EPD0920_2_H11
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: Ifitm2_intron_L1L2_GTK_LacZ_BetactP_neo
• Mutation details: The Ifitm2_intron_L1L2_GTK_LacZ_BetactP_neo cassette was inserted at position 154393803 of Chromosome 2 (Build GRCm39) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 154395140. The critical artificial exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors. (J:155845, J:173534)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 2 lines available
• Carrying any Actl10 Mutation: 13 strains or lines available

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 4 reference(s)