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Osbptm2a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Osbptm2a(EUCOMM)Wtsi
Name: oxysterol binding protein; targeted mutation 2a, Wellcome Trust Sanger Institute
MGI ID: MGI:5473077
Gene: Osbp  Location: Chr19:11965941-11994112 bp, + strand  Genetic Position: Chr19, 8.58 cM
Mutation
origin
Mutant Cell Lines:  EPD0959_2_B12, EPD0959_2_C09, EPD0959_2_C11, EPD0959_2_C12, EPD0959_2_D11, EPD0959_2_D12, EPD0959_2_E11, EPD0959_2_F10, EPD0959_2_G09, EPD0959_2_G11, EPD0959_2_H11, EPD0959_2_H12
Germline Transmission:  Unknown
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 11983522 of Chromosome 19 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 11984802. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Osbp Mutation:  2 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/11/2020
MGI 6.14
The Jackson Laboratory