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Ghrtm1b(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Ghrtm1b(KOMP)Wtsi
Name: growth hormone receptor; targeted mutation 1b, Wellcome Trust Sanger Institute
MGI ID: MGI:5472175
Gene: Ghr  Location: Chr15:3317755-3583352 bp, - strand  Genetic Position: Chr15, 1.84 cM
Mutation
origin
Mutant Cell Line:  EPD0125_5_H05
Germline Transmission:  Earliest citation of germline transmission: J:188991
Parent Cell Line:  JM8.N4 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 3348078 of Chromosome 15 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site was followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of the targeted exon(s) at position 3347243. The critical exon(s) were thus flanked by loxP sites, and subsequent cre excision removed the critical sequence an neomycin selection cassette, yielding a reporter knockout allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)
Expression
In Mice Carrying this Mutation: 23 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Ghr Mutation:  1 strain or line available
References
Original:  J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012;
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/23/2020
MGI 6.15
The Jackson Laboratory