Tg(Nfatc1-rtTA)96Bz
Transgene Detail

Summary

• Symbol: Tg(Nfatc1-rtTA)96Bz
• Name: transgene insertion 96, Bin Zhou
• MGI ID: MGI:5471110
• Synonyms: Nfatc1^nrtTA
• Transgene: Tg(Nfatc1-rtTA)96Bz Location: unknown
• Alliance: Tg(Nfatc1-rtTA)96Bz page

Transgene origin

• Strain of Origin: FVB

Transgene description

• Transgene Type: Transgenic (Transactivator)
• Mutation: Insertion
• Mutation details: Homologous recombination was employed to insert a nuclear-localized reverse tetracycline transactivator sequence and Frt-flanked PGK-neomycin cassette at the translational start site in exon 1 of Nfatc1 in a BAC. The neo cassette was removed with Flpe. The purified construct was injected into fertilized FVB 0.5 day-zygotes. Seven founder lines were obtained and bred to tetO-cre;Gt(ROSA)26Sor^fslz mice, followed by doxycycline induction for 3 days starting on E7.5. Three lines (96, 100, and 122) showed expression in the endocardium and its mesenchymal descendants in the endocardial cushion. Data from #96 is provided in the original reference. (J:193198)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:193198 Wu B, et al., Endocardial cells form the coronary arteries by angiogenesis through myocardial-endocardial VEGF signaling. Cell. 2012 Nov 21;151(5):1083-96
• All: 1 reference(s)