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Targeted Allele Detail
Symbol: Igs5tm1(ACTB-tdTomato,-EGFP)Luo
Name: intergenic site 5; targeted mutation 1, Liqun Luo
MGI ID: MGI:5470394
Synonyms: Iis5tm1(ACTB-tdTomato,-EGFP)Luo, MADM-7TG
Gene: Igs5  Location: unknown  Genetic Position: Chr7, 2.13 cM
Germline Transmission:  Earliest citation of germline transmission: J:194079
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
Mutation detailsThe TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the Iis5 locus locate at about 0.7 kb downstream of exon 5 of the Rps9 gene at 2.13cM on chromosome 7. (J:194079)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Igs5 Mutation:  2 strains or lines available
Original:  J:194079 Hippenmeyer S, et al., Mosaic analysis with double markers reveals cell-type-specific paternal growth dominance. Cell Rep. 2013 Mar 28;3(3):960-7
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory