Steap4^tm1a(KOMP)J
Targeted Allele Detail

Summary

• Symbol: Steap4^tm1a(KOMP)J
• Name: STEAP family member 4; targeted mutation 1a, Jackson
• MGI ID: MGI:5469207
• Gene: Steap4 Location: Chr5:8010472-8032213 bp, + strand Genetic Position: Chr5, 3.37 cM
• Alliance: Steap4^tm1a(KOMP)J page

Mutation origin

• Mutant Cell Lines: JKOMP113781-2G4, JKOMP113781-3F1
• Germline Transmission: Earliest citation of germline transmission: J:188991
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The PG00115_Z_2_H12 targeting vector was obtained from The International Knockout Mouse Consortium by The Jackson Laboratory. The L1L2_Bact_P cassette was inserted at position 7974953 of Chromosome 5 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 7977268. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:148605, J:173534, J:188991)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Steap4 Mutation: 34 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)