Tg(Ikzf1-cre)ACayo
Transgene Detail

Summary

• Symbol: Tg(Ikzf1-cre)ACayo
• Name: transgene insertion A, Michel Cayouette
• MGI ID: MGI:5465164
• Synonyms: IKCre-A
• Transgene: Tg(Ikzf1-cre)ACayo Location: unknown
• Alliance: Tg(Ikzf1-cre)ACayo page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Ikzf1-cre)ACayo expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ikzf1 (MGI:1342540)

• Mutation details: A BAC (RP23-373H2) containing the complete Ikzf1 gene (Ikaros) and large flanking sequences was modified by bacterial recombination to insert a cre-IRES-Venus cassette at the start codon in exon 2. An FRT-flanked neo selection cassette was also inserted. The neo was subsequently removed by Flp expression in bacteria for the final construct. The final construct was used for pronuclear injection to generated transgenic founders. 11 founder lines were obtained but only 9 produced transgenic progeny. Three founder lines (A,F, and G) were chosen for further analyses after crosses to reporter mice to assess recombination profiles. Line A labels dense arrays of retinal progenitor cells. (J:191133)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ikzf1 (mouse) Summary of all recombinase alleles driven by Ikzf1. Alliance mouse Ikzf1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:191133 Tarchini B, et al., In vivo evidence for unbiased Ikaros retinal lineages using an Ikaros-Cre mouse line driving clonal recombination. Dev Dyn. 2012 Dec;241(12):1973-85
• All: 3 reference(s)