Tg(Cyp11a1-icre)1Aswn
Transgene Detail

Summary

• Symbol: Tg(Cyp11a1-icre)1Aswn
• Name: transgene insertion 1, Amanda Swain
• MGI ID: MGI:5462056
• Synonyms: Cyp11a1-Cre
• Transgene: Tg(Cyp11a1-icre)1Aswn Location: unknown
• Alliance: Tg(Cyp11a1-icre)1Aswn page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Recombinase)
• Mutation: Insertion
• Tg(Cyp11a1-icre)1Aswn expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	Cyp11a1

• Mutation details: A targeting construct was generated with an improved cre coding region and rabbit beta-globin poly A signal sequence inserted into the 5' UTR of Cyp11a1, 14 bp upstream of the ATG codon. The construct contained a 2.8 kb Cyp11a1 fragment with the cre/poly A insertion (with a 100 bp deletion in Cyp11a1, including the ATG site). This fragment was introduced into bacterial cells for homologous recombination with a BAC containing the Cyp11a1 gene sequence. Recombined BAC DNA was purified for injection into fertilized pronuclei. One founder line was characterized by maing with two different mouse strains carrying cre-responsive reporters. (J:189958)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Cyp11a1 (species not specified) Summary of all recombinase alleles driven by Cyp11a1.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:189958 Buaas FW, et al., In vivo evidence for the crucial role of SF1 in steroid-producing cells of the testis, ovary and adrenal gland. Development. 2012 Dec;139(24):4561-70
• All: 2 reference(s)