Hdc^tm2.1Wwis
Targeted Allele Detail

Summary

• Symbol: Hdc^tm2.1Wwis
• Name: histidine decarboxylase; targeted mutation 2.1, William Wisden
• MGI ID: MGI:5461703
• Synonyms: HDC-EGFP
• Gene: Hdc Location: Chr2:126435587-126461219 bp, - strand Genetic Position: Chr2, 61.76 cM, cytoband E5-G
• Alliance: Hdc^tm2.1Wwis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:188103
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector used to generate the initial Hdc-cre allele was modified such that an IRES2i-EGFP-IRES2i-cre-FRT-neo-FRT sequence was inserted into exon 12 of Hdc after the stop codon. The resulting allele is tricistronic producing an mRNA coding for Hdc, EGFP and cre. Breeding to Flp deleter mice removed the neo marker. However, cre is not translated sufficiently from this mRNA configuration to result in recombination of floxed gene sequences. (J:188103)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hdc Mutation: 50 strains or lines available

References

• Original: J:188103 Zecharia AY, et al., GABAergic inhibition of histaminergic neurons regulates active waking but not the sleep-wake switch or propofol-induced loss of consciousness. J Neurosci. 2012 Sep 19;32(38):13062-75
• All: 1 reference(s)