Mrgprb4^tm2.1And
Targeted Allele Detail

Summary

• Symbol: Mrgprb4^tm2.1And
• Name: MAS-related GPR, member B4; targeted mutation 2.1, David J Anderson
• MGI ID: MGI:5448560
• Synonyms: MrgprB4-Tta-IRES-hPlap-ACN
• Gene: Mrgprb4 Location: Chr7:47847961-47848926 bp, - strand Genetic Position: Chr7, 31.11 cM
• Alliance: Mrgprb4^tm2.1And page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The entire open reading frame of the gene, encoded by a single exon, was replaced with a tTA-IRES-hPLAP-ACN targeting cassette. Tetracycline transactivator (Tta), an internal ribosome entry site (IRES), and human placental alkaline phosphatase (hPLAP) are fused with a self-excising loxP-flanked pol-II promoter-neomycin resistance cassette (ACN). Cre-mediated recombination removed the neo cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mrgprb4 Mutation: 25 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 2 reference(s)