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Targeted Allele Detail
Symbol: Raf1tm2.1Ara
Name: v-raf-leukemia viral oncogene 1; targeted mutation 2.1, Toshiyuki Araki
MGI ID: MGI:5445964
Synonyms: Raf1D486N
Gene: Raf1  Location: Chr6:115618067-115676635 bp, - strand  Genetic Position: Chr6, 53.62 cM, cytoband C3
Germline Transmission:  Earliest citation of germline transmission: J:189143
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Allele Type:    Targeted (Not Specified)
Mutations:    Insertion, Single point mutation
Mutation detailsA targeting vector containing a loxP site, cDNA spanning exons 13 through 16, neo cassette, second loxP site and modified exon 13 was inserted into intron 12. The modified exon 13 contains nucleotide substitution(s) that result in the amino acid substitution of asparagine for aspartic acid at position 486 (D486N). Cre-mediated recombination removed the cDNA and neo cassette. (J:189143)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Raf1 Mutation:  102 strains or lines available
Original:  J:189143 Wu X, et al., Increased BRAF heterodimerization is the common pathogenic mechanism for noonan syndrome-associated RAF1 mutants. Mol Cell Biol. 2012 Oct;32(19):3872-90
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.12
The Jackson Laboratory