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Transgene Detail
Symbol: Tg(ACTA1-TPM3*M9R)4Hrd
Name: transgene insertion 4, Edna Hardeman
MGI ID: MGI:5444208
Synonyms: HSA-alphaTmslow(Met9Arg), HSA-alphaTmslow(Met9Arg), HSA-aTmslow(Met9Arg), HSA-aTmslow(Met9Arg), HSA-TPM3*M9R, Tg(TPM3)Met9Arg, TPM3(Met9Arg)
Transgene: Tg(ACTA1-TPM3*M9R)4Hrd  Location: unknown  
Strain of Origin:  FVB/NJ
Transgene Type:    Transgenic (Dominant negative, Humanized sequence, Inserted expressed sequence)
Mutation:    Insertion
Tg(ACTA1-TPM3*M9R)4Hrd expresses 1 gene
Mutation detailsFirst, the human tropomyosin 3 gene was used to isolate a full-length alphaTmslow cDNA sequence. Next, the sequence was modified by site-directed mutagenesis to have the ATG-to-AGG base pair substitution encoding a methionine-to-arginine substitution at codon 9 (M9R); resulting in the dominant-negative alphaTmslow(Met9Arg) mutation associated with human nemaline myopathy. The mutant cDNA sequence was placed downstream of a 2.2 kbp human alpha-skeletal actin (ACTA1) promoter sequence, and upstream of a 1022 bp cassette containing the SV40 small t antigen intron and 3' untranslated region. Lines 4, 9 and 14 were generated with high, low and moderate expression levels, respectively. (J:67596)
View phenotypes for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Original:  J:67596 Corbett MA, et al., A mutation in alpha-tropomyosin(slow) affects muscle strength, maturation and hypertrophy in a mouse model for nemaline myopathy. Hum Mol Genet. 2001 Feb 15;10(4):317-28
All:  3 reference(s)

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MGI 6.01
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