Col1a1^{tm4(CAG-luc2)Nki}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm4(CAG-luc2)Nki}
• Name: collagen, type I, alpha 1; targeted mutation 4, The Netherlands Cancer Institute
• MGI ID: MGI:5443879
• Synonyms: Col1A1^{tm4(lox66-Cag-lox71-Luc2)Nki}, frt-invCag-Luc
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm4(CAG-luc2)Nki} page

Mutation origin

• Mutant Cell Lines: 1.5_1B1_6, 1.5_1B1_9, 1.5_1B1_11
• Germline Transmission: Earliest citation of germline transmission: J:210103
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: FVB;129P2-Trp53 Col1A1 Rb1 were retargeted to replace the frt-flanked PGK-Neo cassette in the 3' UTR of the Col1a1 by a "Flp-in" plasmid encoding a frt-lox66-Cag-lox71-Luc2 inversion transgene, called inv-Cag-Luc2, by co-transfected with a Flpe-expressing plasmid to facilitate recombination into the 3' UTR of the Col1a1 locus. This transgene contains the constitutive CAGGs promoter that drives the expression of the Hygromycin-resistance gene, present in the targeted Col1a1 locus. Upon expression of the Cre recombinase, the promoter is inverted due to flanking mutant loxP sites, i.e. lox66 and lox71, placing the promoter in front of the firefly Luciferase2 gene. (J:188992, J:210103)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

ES cells contained Trp53^tm1Brn and Rb1^tm2Brn in addition to the targeted Col1a1^tm2Nki allele.

References

• Original: J:188992 The Netherlands Cancer Institute, Direct data submission by The Netherlands Cancer Institute. MGI Direct Data Submission. 2012
• All: 3 reference(s)