Col1a1^tm3Nki
Targeted Allele Detail

Summary

• Symbol: Col1a1^tm3Nki
• Name: collagen, type I, alpha 1; targeted mutation 3, The Netherlands Cancer Institute
• MGI ID: MGI:5443866
• Synonyms: Col1a1^{tm3(frt-PGK-neo-frt-deltaATGHygro)Nki}, Col1a1^{tm3(frt-PGK-neo-frt)Nki}
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^tm3Nki page

Mutation origin

• Mutant Cell Lines: 1.1_1E5, 1.1_1F5, 1.1_1F6, 1.1_1F6_s6
• Germline Transmission: Earliest citation of germline transmission: J:210103
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The ES cells were targeted in the 3' UTR of the Col1a1 locus with a Flp-in construct containing a frt flanked PGK-Neo cassette followed by a Hygromicin gene lacking a proper ATG. Correctly targeted ES cells were selected on basis of Neomycin resistance. The targeted Col1a1 locus can later be used as a docking site for the introduction of a plasmid, minimally containing the desired transgene sequence and an frt site preceded by a constitutive promoter and an ATG initiation codon. (J:188992, J:210103)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 160 strains or lines available

Notes

Parental ES cells contain Cdkn2a^tm1.1Brn Nf2^tm2Gth Trp53^tm1Brn mice.

References

• Original: J:188992 The Netherlands Cancer Institute, Direct data submission by The Netherlands Cancer Institute. MGI Direct Data Submission. 2012
• All: 2 reference(s)