Pkhd1^tm2.1Cjwa
Targeted Allele Detail

Summary

• Symbol: Pkhd1^tm2.1Cjwa
• Name: polycystic kidney and hepatic disease 1; targeted mutation 2.1, Christopher J Ward
• MGI ID: MGI:5438325
• Synonyms: Pkhd1^Pk(+)
• Gene: Pkhd1 Location: Chr1:20128003-20688288 bp, - strand Genetic Position: Chr1, 6.27 cM, cytoband A2-A5
• Alliance: Pkhd1^tm2.1Cjwa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:187735
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Pkhd1^tm2Cjwa mice were crossed with Tg(Gdf9-cre)5092Coo mice to remove the LSL (loxP-STOP-loxP) cassette leaving a single loxP site in intron 2. Upon removal of the LSL, the gene is reactivated and the expressed protein carries two SV5-Pk epitope tags on its extreme N-terminus. Northern blot analysis reveals the presence of 13 kb, 9 kb, 7.7kb and 7.5kb products identical in length to products generated from the wild-type allele. (J:187735)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pkhd1 Mutation: 222 strains or lines available

References

• Original: J:187735 Bakeberg JL, et al., Epitope-tagged Pkhd1 tracks the processing, secretion, and localization of fibrocystin. J Am Soc Nephrol. 2011 Dec;22(12):2266-77
• All: 1 reference(s)