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Targeted Allele Detail
Symbol: Pkhd1tm2Cjwa
Name: polycystic kidney and hepatic disease 1; targeted mutation 2, Christopher J Ward
MGI ID: MGI:5438324
Synonyms: Pkhd1LSL(-)
Gene: Pkhd1  Location: Chr1:20057779-20618064 bp, - strand  Genetic Position: Chr1, 6.27 cM, cytoband A2-A5
Germline Transmission:  Earliest citation of germline transmission: J:187735
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
Mutation detailsThe targeting vector was designed with a loxP-STOP-loxP (LSL) cassette containing a puromycin acetyltransferase gene (pac) and 4 copies of the SV40 viral transcriptional termination sequence inserted into intron 2 and two SV5-Pk epitope tags (26 amino acids long) inserted in-frame into exon 3. The LSL cassette terminates all transcripts in intron 2 generating a null allele. Northern blotting and RT-PCR analysis confirmed the absence of mRNA. (J:187735)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Pkhd1 Mutation:  150 strains or lines available
Original:  J:187735 Bakeberg JL, et al., Epitope-tagged Pkhd1 tracks the processing, secretion, and localization of fibrocystin. J Am Soc Nephrol. 2011 Dec;22(12):2266-77
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.11
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