Diaph1^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Diaph1^{tm1a(EUCOMM)Hmgu}
• Name: diaphanous related formin 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5437924
• Gene: Diaph1 Location: Chr18:37976654-38068529 bp, - strand Genetic Position: Chr18, 19.71 cM
• IMPC: Diaph1 gene page

Mutation origin

• Mutant Cell Lines: HEPD0837_3_A10, HEPD0837_3_B09, HEPD0837_3_B10, HEPD0837_3_B11, HEPD0837_3_B12, HEPD0837_3_C09, HEPD0837_3_C10, HEPD0837_3_C12, HEPD0837_3_D09, HEPD0837_3_D10, HEPD0837_3_D11, HEPD0837_3_D12, HEPD0837_3_E09, HEPD0837_3_E10, HEPD0837_3_E12, HEPD0837_3_F09, HEPD0837_3_F10, HEPD0837_3_F11, HEPD0837_3_G09, HEPD0837_3_G12, HEPD0837_3_H10, HEPD0837_3_H11
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_st2
• Mutation details: The L1L2_st2 cassette was inserted at position 38034891 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 38035644. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Diaph1 Mutation: 117 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)