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Il13tm2.1Lky
Targeted Allele Detail
Nomenclature
Symbol: Il13tm2.1Lky
Name: interleukin 13; targeted mutation 2.1, Richard M Locksley
MGI ID: MGI:5435899
Synonyms: Il13Smart, IL-13Smart, Il13tm2.1(CD4)Lky, Smart13
Gene: Il13  Location: Chr11:53522151-53525529 bp, - strand  Genetic Position: Chr11, 31.98 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:178986
Parent Cell Line:  PC3 (ES Cell)
Strain of Origin:  129S4/SvJae-Tg(Prm-cre)70Og
Mutation
description
Allele Type:    Targeted (Reporter)
Mutation:    Insertion
 
Mutation detailsA targeting vector was designed to insert a neomycin (neo) resistance cassette, and an internal ribosome entry site (IRES), followed by truncated human CD4 cDNA, between the stop codon and the 3' untranslated region. The truncated CD4 fragment(424 aa vs. 458 aa in full-length protein) lacking intracellular signaling capacity (aa 419-424 as cytoplasmic tail in truncated form vs. aa 419-458 in full-length hCD4), and also contains a point mutation resulting in an I(ATC codon) to F(TTC codon) substitution at position 43 of the protein, rendering the human CD4 marker incapable of interacting with mouse major histocompatibility complex class II (MHCII) and useful as a marker of cells expressing IL13. Cre-mediated recombination removed the neo cassette. (J:178986)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Il13 Mutation:  23 strains or lines available
References
Original:  J:178986 Liang HE, et al., Divergent expression patterns of IL-4 and IL-13 define unique functions in allergic immunity. Nat Immunol. 2011;13(1):58-66
All:  16 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/30/2021
MGI 6.17
The Jackson Laboratory