Avil^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Avil^{tm1a(EUCOMM)Hmgu}
• Name: advillin; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5435790
• Gene: Avil Location: Chr10:126836578-126856863 bp, + strand Genetic Position: Chr10, 74.48 cM, cytoband D3
• IMPC: Avil gene page

Mutation origin

• Mutant Cell Lines: HEPD0832_9_A01, HEPD0832_9_A04, HEPD0832_9_A06, HEPD0832_9_A11, HEPD0832_9_B07, HEPD0832_9_C02, HEPD0832_9_C10, HEPD0832_9_C11, HEPD0832_9_D02, HEPD0832_9_D03, HEPD0832_9_E05, HEPD0832_9_E10, HEPD0832_9_F01, HEPD0832_9_F05, HEPD0832_9_F06, HEPD0832_9_G01, HEPD0832_9_G03, HEPD0832_9_H02, HEPD0832_9_H08, HEPD0837_8_A01, HEPD0837_8_A04, HEPD0837_8_A06, HEPD0837_8_A08, HEPD0837_8_B01, HEPD0837_8_B02, HEPD0837_8_B03, HEPD0837_8_B04, HEPD0837_8_B06, HEPD0837_8_B07, HEPD0837_8_B08, HEPD0837_8_B12, HEPD0837_8_C01, HEPD0837_8_C03, HEPD0837_8_C04, HEPD0837_8_C05, HEPD0837_8_C07, HEPD0837_8_C10, HEPD0837_8_C12, HEPD0837_8_D01, HEPD0837_8_D02, HEPD0837_8_D04, HEPD0837_8_D06, HEPD0837_8_D07, HEPD0837_8_D09, HEPD0837_8_D10, HEPD0837_8_E03, HEPD0837_8_E12, HEPD0837_8_F01, HEPD0837_8_F03, HEPD0837_8_F04, HEPD0837_8_F06, HEPD0837_8_F09, HEPD0837_8_F10, HEPD0837_8_F11, HEPD0837_8_F12, HEPD0837_8_G04, HEPD0837_8_G06, HEPD0837_8_G07, HEPD0837_8_G12, HEPD0837_8_H01, HEPD0837_8_H03, HEPD0837_8_H04, HEPD0837_8_H06, HEPD0837_8_H09, HEPD0837_8_H10, HEPD0837_8_H12
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 126841943 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 126842835. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Avil Mutation: 45 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)