Sec23b^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sec23b^{tm1b(EUCOMM)Wtsi}
• Name: SEC23 homolog B, COPII coat complex component; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5433325
• Gene: Sec23b Location: Chr2:144398165-144432673 bp, + strand Genetic Position: Chr2, 71.02 cM, cytoband H1
• Alliance: Sec23b^{tm1b(EUCOMM)Wtsi} page
• IMPC: Sec23b gene page

Mutation origin

• Mutant Cell Line: EPD0237_3_G06
• Germline Transmission: Earliest citation of germline transmission: J:186391
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 144408239 of Chromosome 2 upstream of the exons 5 and 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 6 at position 144409359. The critical exons are thus flanked by loxP sites. Cre expression removes exons 5 and 6 creating a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:186391)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sec23b Mutation: 49 strains or lines available

References

• Original: J:186391 Tao J, et al., SEC23B is required for the maintenance of murine professional secretory tissues. Proc Natl Acad Sci U S A. 2012 Jul 17;109(29):E2001-9
• All: 1 reference(s)