Nsa2^{tm1e(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Nsa2^{tm1e(EUCOMM)Hmgu}
• Name: NSA2 ribosome biogenesis homolog; targeted mutation 1e, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5432743
• Gene: Nsa2 Location: Chr13:97265935-97274434 bp, - strand Genetic Position: Chr13, 50.66 cM, cytoband D1
• IMPC: Nsa2 gene page

Mutation origin

• Mutant Cell Lines: HEPD0824_3_A01, HEPD0824_3_B03, HEPD0824_3_C02, HEPD0824_3_C03, HEPD0824_3_D01, HEPD0824_3_E02, HEPD0824_3_E03, HEPD0824_3_G01, HEPD0824_3_G02, HEPD0824_3_G04, HEPD0824_5_B05, HEPD0824_5_B06, HEPD0824_5_B08, HEPD0824_5_C06, HEPD0824_5_C07, HEPD0824_5_C08, HEPD0824_5_D07, HEPD0824_5_E07, HEPD0824_5_E08, HEPD0824_5_F07, HEPD0824_5_F08, HEPD0824_5_G07, HEPD0824_5_G08, HEPD0824_5_H05
• Germline Transmission: Unknown
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 97272688 of Chromosome 13 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression will remove the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nsa2 Mutation: 13 strains or lines available

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 1 reference(s)