Prkch^tm1.2Gasc
Targeted Allele Detail

Summary

• Symbol: Prkch^tm1.2Gasc
• Name: protein kinase C, eta; targeted mutation 1.2, Nicholas Gascoigne
• MGI ID: MGI:5431008
• Gene: Prkch Location: Chr12:73631570-73824959 bp, + strand Genetic Position: Chr12, 30.81 cM, cytoband C3-D1
• Alliance: Prkch^tm1.2Gasc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:186080
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A targeting vector was designed to insert a loxP site, followed by a frt-flanked neomycin resistance (neo) cassette, upstream of exon 2 and a second loxP site downstream of exon 2. Flp-mediated recombination removed the neo cassette while cre-mediated recombination remvoed exon 2. (J:186080)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkch Mutation: 40 strains or lines available

References

• Original: J:186080 Fu G, et al., Protein kinase C eta is required for T cell activation and homeostatic proliferation. Sci Signal. 2011;4(202):ra84
• All: 5 reference(s)