Tg(CAG-Bgeo,-SMN2)E9Dscd
Transgene Detail

Summary

• Symbol: Tg(CAG-Bgeo,-SMN2)E9Dscd
• Name: transgene insertion E9, Dawn S Chandler
• MGI ID: MGI:5427354
• Synonyms: Tg hSMN2 E9
• Transgene: Tg(CAG-Bgeo,-SMN2)E9Dscd Location: unknown
• Alliance: Tg(CAG-Bgeo,-SMN2)E9Dscd page

Transgene origin

• Germline Transmission: Earliest citation of germline transmission: J:185111
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Transgene description

• Transgene Type: Transgenic (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The pCCALL2/hSMN2 transgene was designed with, from 5' to 3', the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (originally from the pCAGG vector), a loxP-flanked STOP cassette (beta-galactosidase/neomycin resistance cassette [beta-geo] with three copies of the SV40 polyA signal), the 1.37 kbp full-length human SMN2 cDNA sequence including all exons (1-8) from the start codon to the stop codon, and a rabbit polyA signal. The 1.37 kbp human SMN2 cDNA sequence (encoding exons 1-8) was originally derived via RT-PCR performed on total RNA isolated from SMA type 1-derived patient fibroblast cells (Coriell Institute GM 3813; shown to express both full-length and truncated SMN2 transcripts). Lines A9 and E9 was generated. (J:185111)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:185111 Bebee TW, et al., Generation of a tamoxifen inducible SMN mouse for temporal SMN replacement. Genesis. 2011 Dec;49(12):927-34
• All: 1 reference(s)