Mir702^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir702^tm1Wtsi
• Name: microRNA 702; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5320155
• Synonyms: Mir702^{tm1(mirKO)Wtsi}
• Gene: Mir702 Location: Chr5:137020287-137020395 bp, + strand Genetic Position: Chr5, 76.09 cM

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_1F1, mirKO_ES_PuDtk_1F2, mirKO_ES_PuDtk_1F3, mirKO_ES_PuDtk_1F4, mirKO_ES_PuDtk_1F5, mirKO_ES_PuDtk_1F6, mirKO_ES_PuDtk_1F7, mirKO_ES_PuDtk_1F8, mirKO_ES_PuDtk_1F9, mirKO_ES_PuDtk_1F10, mirKO_ES_PuDtk_1F11, mirKO_ES_PuDtk_1F12
• Germline Transmission: Unknown
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 162 starting at position 137020280 and ending at position 137020442 of Chromosome 5 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir702 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)