Mir210^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir210^tm1Wtsi
• Name: microRNA 210; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5320152
• Synonyms: Mir210^{tm1(mirKO)Wtsi}
• Gene: Mir210 Location: Chr7:140801297-140801406 bp, - strand Genetic Position: Chr7, 86.52 cM
• Alliance: Mir210^tm1Wtsi page

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_1A1, mirKO_ES_PuDtk_1A2, mirKO_ES_PuDtk_1A3, mirKO_ES_PuDtk_1A11, mirKO_ES_PuDtk_1A12, mirKO_ES_PuDtk_1B1, mirKO_ES_PuDtk_1B2, mirKO_ES_PuDtk_1B3, mirKO_ES_PuDtk_1B6, mirKO_ES_PuDtk_1B8, mirKO_ES_PuDtk_1B9, mirKO_ES_PuDtk_1B10
• Germline Transmission: Earliest citation of germline transmission: J:205868
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 237 starting at position 140801235 and ending at position 140801472 of Chromosome 7 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir210 Mutation: 8 strains or lines available

Notes

Cell line mirKO_ES_PuDtk_1A1 was successfully used to make chimeric mice. Germline transmission was accomplished. J:205868

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)