Mir483^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir483^tm1Wtsi
• Name: microRNA 483; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5320151
• Synonyms: Mir483^{tm1(mirKO)Wtsi}
• Gene: Mir483 Location: Chr7:142208661-142208733 bp, - strand Genetic Position: Chr7, 87.99 cM
• Alliance: Mir483^tm1Wtsi page

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_1H7, mirKO_ES_PuDtk_1H8, mirKO_ES_PuDtk_1H9, mirKO_ES_PuDtk_1H10, mirKO_ES_PuDtk_1H11, mirKO_ES_PuDtk_1H12
• Germline Transmission: Earliest citation of germline transmission: J:298625
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 135 starting at position 142208657 and ending at position 142208792 of Chromosome 7 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir483 Mutation: 2 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)