Mir219a-2^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir219a-2^tm1Wtsi
• Name: microRNA 219a-2; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5320146
• Synonyms: Mir219a-2^{tm1(mirKO)Wtsi}
• Gene: Mir219a-2 Location: Chr2:29735643-29735739 bp, - strand Genetic Position: Chr2, 20.72 cM

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_1C7, mirKO_ES_PuDtk_1C8, mirKO_ES_PuDtk_1C9, mirKO_ES_PuDtk_1C10, mirKO_ES_PuDtk_1C11, mirKO_ES_PuDtk_1C12
• Germline Transmission: Unknown
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 186 starting at position 29735590 and ending at position 29735776 of Chromosome 2 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir219a-2 Mutation: 4 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)