Mir300^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mir300^tm1Wtsi
• Name: microRNA 300; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5320107
• Synonyms: Mir300^{tm1(mirKO)Wtsi}
• Gene: Mir300 Location: Chr12:109690747-109690825 bp, + strand Genetic Position: Chr12, 60.56 cM

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_15D1, mirKO_ES_PuDtk_15D2, mirKO_ES_PuDtk_15D4, mirKO_ES_PuDtk_15D5, mirKO_ES_PuDtk_15D6
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mutation details: The insertion of the PGK_EM7_PuDtk_bGHpA cassette created a deletion of size 186 starting at position 109690710 and ending at position 109690896 of Chromosome 12 (Build GRCm39). This deletion eliminates the DNA sequence for this microRNA. The cassette is composed of a loxP site followed by an F3 site followed by a PGK-puromycin-delta-tk cassette, a loxP site and finally an FRT site. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir300 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)