About   Help   FAQ
Targeted Allele Detail
Symbol: Igs1tm5(CAG-Bgeo,-Mito-GFP)Nat
Name: intergenic site 1; targeted mutation 5, Jeremy Nathans
MGI ID: MGI:5319536
Synonyms: Iis1tm5(CAG-Bgeo,-Mito-GFP)Nat, Iis1tm5(CAG-Bgeo,-Mito-GFP/V5)Nat, Ubbtm1(Mito-GFP-V5)Nat
Gene: Igs1  Location: unknown  Genetic Position: Chr11, Syntenic
Germline Transmission:  Earliest citation of germline transmission: J:101977
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129
Allele Type:    Targeted (Conditional ready, No functional change, Reporter)
Mutation:    Insertion
Mutation detailsThe following elements were inserted, in order, 2 kb 5' of the Ubb gene by targeted recombination in 129-derived ES cells: CMV enhancer/beta-actin promoter (CAG), loxP site, beta-geo, 3 transcription sites, loxP site, mitochondria targeted GFP fused to a C-terminal V5 epitope, and a bovine growth hormone 3' UTR. Mitochondrial targeting was achieved by using the N-terminal 25 amino acid targeting signal of mouse Cox8a. The V5 epitope encodes amino acid residues 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5. (J:101977)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Igs1 Mutation:  10 strains or lines available
Original:  J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.16
The Jackson Laboratory