Slc26a5^{tm4.1(cre/ERT2)Jnz}
Targeted Allele Detail

Summary

• Symbol: Slc26a5^{tm4.1(cre/ERT2)Jnz}
• Name: solute carrier family 26, member 5; targeted mutation 4.1, Jian Zou
• MGI ID: MGI:5316512
• Synonyms: prestin-CreER^T2-NN
• Gene: Slc26a5 Location: Chr5:22015653-22070602 bp, - strand Genetic Position: Chr5, 9.97 cM, cytoband A3
• Alliance: Slc26a5^{tm4.1(cre/ERT2)Jnz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:182720
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Slc26a5^{tm4.1(cre/ERT2)Jnz} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Slc26a5 (MGI:1933154)

• Mutation details: A targeting vector was generated using recombineering methods to modify a BAC containing the Slc26a5 (prestin) locus. An IRES-cre/ERT2-frt-neo-frt cassette was inserted after the stop codon in exon 20. The vector was electroporated into TL-1 ES cells to produce knockin mice. Mice were crossed with Tg(ACTFlpe)9205Dym animals to delete the neo cassette. (J:182720)

Generation of Slc26a5^{tm4(cre/ERT2)Jnz} and Slc26a5^{tm4.1(cre/ERT2)Jnz} alleles.

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Slc26a5 (mouse) Summary of all recombinase alleles driven by Slc26a5. Alliance mouse Slc26a5 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc26a5 Mutation: 54 strains or lines available

References

• Original: J:182720 Fang J, et al., Outer hair cell-specific prestin-CreERT2 knockin mouse lines. Genesis. 2012 Feb;50(2):124-31
• All: 8 reference(s)