Mirc22^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc22^tm1Wtsi
• Name: microRNA cluster 22, including Mir23b through Mir24-1; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5315160
• Synonyms: Mir23b-Mir24-1 cluster 13N5, Mir23b^{tm1(mirKO)Wtsi}
• Gene: Mirc22 Location: Chr13:63448298-63449089 bp Genetic Position: Chr13, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_15A7, mirKO_ES_PuDtk_22C1, mirKO_ES_PuDtk_22C2, mirKO_ES_PuDtk_22C4, mirKO_ES_PuDtk_22C5, mirKO_ES_PuDtk_22C6
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: Mir23b, Mir27b, and Mir24-1 in this microRNA cluster were replaced via homologous recombination with a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters. The inserted selection cassette was flanked by LoxP and F3/FRT sites. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc22 Mutation: 3 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 1 reference(s)