Mirc20^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc20^tm1Wtsi
• Name: microRNA cluster 20, including Mir302b through Mir367; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5315157
• Synonyms: Mir302b-Mir367 cluster 3N2, Mir302b^{tm1(mirKO)Wtsi}
• Gene: Mirc20 Location: Chr3:127338877-127339456 bp Genetic Position: Chr3, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_13F1, mirKO_ES_PuDtk_13F2, mirKO_ES_PuDtk_13F3, mirKO_ES_PuDtk_13F4, mirKO_ES_PuDtk_13F5
• Germline Transmission: Earliest citation of germline transmission: J:228522
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion Vector: PGK_EM7_PuDtk_bGHpA
• Mirc20^tm1Wtsi involves 6 genes/genome features (Mir302b, Mirc20, Mir302c ...) View all
• Mutation details: The microRNA cluster including Mir302b, Mir302c, Mir302a, Mir302d and Mir367 was replaced via homologous recombination with a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters. The inserted selection cassette was flanked by LoxP and F3/FRT sites. (J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc20 Mutation: 1 strain or line available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)