Mirc15^tm1Wtsi
Targeted Allele Detail

Summary

• Symbol: Mirc15^tm1Wtsi
• Name: microRNA cluster 15, including Mir181a-2 through Mir181b-2; targeted mutation 1, Wellcome Trust Sanger Institute
• MGI ID: MGI:5315151
• Synonyms: Mir181a-2/Mir181b-2 cluster 2N1, Mir181a-2^{tm1(mirKO)Wtsi}, Mirc15^{tm1(mirKO)Wtsi}
• Gene: Mirc15 Location: Chr2:38742747-38743930 bp Genetic Position: Chr2, Syntenic

Mutation origin

• Mutant Cell Lines: mirKO_ES_PuDtk_13E1, mirKO_ES_PuDtk_13E2, mirKO_ES_PuDtk_13E3
• Germline Transmission: Unknown
• Parent Cell Line: JM8A3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: Sanger miRNA knockouts

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion
• Mutation details: The microRNA cluster including Mir181a-2 and Mir181b-2 was replaced via homologous recombination with a puromycin/thymidine kinase fusion gene under the control of EM7/PGK promoters. The inserted selection cassette was flanked by LoxP and F3/FRT sites. (J:94077, J:174268)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mirc15 Mutation: 2 strains or lines available

References

• Original: J:174268 Prosser HM, et al., A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nat Biotechnol. 2011 Aug 7;29(9):840-5
• All: 2 reference(s)