Tg(TcraCWM5,TcrbCWM5)1807Wuth
Transgene Detail

Summary

• Symbol: Tg(TcraCWM5,TcrbCWM5)1807Wuth
• Name: transgene insertion 1807, Marcel Wuethrich
• MGI ID: MGI:5315014
• Synonyms: Bd1807, Bd 1807 TCR Tg, Blastomyces-specific TCR Tg 1807
• Transgene: Tg(TcraCWM5,TcrbCWM5)1807Wuth Location: unknown
• Alliance: Tg(TcraCWM5,TcrbCWM5)1807Wuth page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TcraCWM5,TcrbCWM5)1807Wuth expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: The CW/M 5 CD4+ T cell line is from C57BL/6 mice injected with cell wall/membrane (CW/M) of Blastomyces dermatitidis yeast cells. The identity of the T cell receptor (TCR) Valpha and Vbeta chains on the surface of the "CW/M 5" T cells was found to be Valpha2 Jalpha49 (amino acid motif of the CDR3 region is AGGYQNFYFGKGSLTVIP) and Vbeta1/D/Jbeta1.1 (amino acid motif of the CDR3 region is SQEGTGG). The TCR-alpha gene segment, containing the rearranged VJ region from 7 bp upstream of the start codon to 71 bp beyond the Jalpha49 coding region into the JC intron, was amplified from genomic DNA of clone #5. This fragment was cloned into the pTalpha cassette. In a similar manner, the TCR-beta gene segment containing the rearranged VDJ region from 16 bp upstream of the start codon to 17 bp beyond the Jbeta1.1 coding regions in the JC intron, was cloned into the pTbeta cassette. The resulting constructs were co-microinjected into C57BL/6 oocytes. Linkage PCR determined that the transgenes in line 1807 had co-integrated in a TCRbeta-TCRalpha head-to-tail and TCRalpha-TCRbeta tail-to-tail fashion. (J:182207)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:182207 Wuthrich M, et al., Fungi subvert vaccine T cell priming at the respiratory mucosa by preventing chemokine-induced influx of inflammatory monocytes. Immunity. 2012 Apr 20;36(4):680-92
• All: 11 reference(s)