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Igs3tm1.1(ACTB-EGFP*)Luo
Targeted Allele Detail
Nomenclature
Symbol: Igs3tm1.1(ACTB-EGFP*)Luo
Name: intergenic site 3; targeted mutation 1.1, Liqun Luo
MGI ID: MGI:5314259
Synonyms: Iis3tm1.1(ACTB-EGFP*)Luo, M10TG, Miya10TG, Tg(ACTB-EGFP*)10Luo
Gene: Igs3  Location: unknown  Genetic Position: Chr10, Syntenic
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:181830
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutation:    Insertion
 
Mutation detailsThe pMADMalpha transgene was designed with the CMV enhancer/chicken beta-actin core promoter (pCA) promoter, a frt site, the MADM GT cassette, and second frt site, the MADM TG cassette, a third frt site, and an SV40 T-antigen poly(A) signal. The MADM GT cassette used here has the N-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP first exon; nucleotides 1-274), a beta-globin intronic sequence (containing a loxP-flanked neomycin resistance gene), and ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus (tdT3MycATG-less). The MADM TG cassette used here has an ATG start codon, a beta-globin intronic sequence (containing a loxP-flanked neomycin resistance gene), and the C-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP second exon; nucleotides 275-735 including stop codon). The transgene was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells were screened for intact single-copy transgenes with integration in intergenic regions close to any centromere. ES cells were identified with an intact single-copy of the transgene inserted into an intergenic region at ~21 Mbp on chromosome 10 between the Ahi1 and Myb loci; this new insertion locus was named Miya10 (or M10). The transgene inserted into an intergenic region at ~21 Mbp on chromosome 10 between the Ahi1 and Myb loci; this new insertion locus was named Miya10 (or M10). ]Cre-mediated recombination removed the neo cassette. (J:181830)
Generation of the Igs3tm1.1(ACTB-EGFP*)Luo and Igs3tm2.1(ACTB-EGFP*,-tdTomato)Luo alleles
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Igs3 Mutation:  2 strains or lines available
References
Original:  J:181830 Tasic B, et al., Extensions of MADM (mosaic analysis with double markers) in mice. PLoS One. 2012;7(3):e33332
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
04/13/2021
MGI 6.16
The Jackson Laboratory