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Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo
Targeted Allele Detail
Nomenclature
Symbol: Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 7, Liqun Luo
MGI ID: MGI:5314253
Synonyms: R26TG
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:181830
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsA targeting vector was created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "MADM TG" cassette. The "MADM TG" cassette used here (ATG-intron-GFPC-terminus) was designed with an ATG start codon, a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] and then a loxP-flanked neomycin resistance gene), the C-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP second exon; nucleotides 275-735 including stop codon), and an SV40 T-antigen poly(A) signal. This entire construct was subcloned into the pROSA26-PA targeting vector to generate the final targeting construct pROSA26-TG. The pROSA26-TG construct was inserted into the Gt(ROSA)26Sor locus. (J:181830)
Generation of various Gt(ROSA)26Sor alleles
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  538 strains or lines available
References
Original:  J:181830 Tasic B, et al., Extensions of MADM (mosaic analysis with double markers) in mice. PLoS One. 2012;7(3):e33332
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
09/15/2020
MGI 6.15
The Jackson Laboratory