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Targeted Allele Detail
Symbol: Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 6, Liqun Luo
MGI ID: MGI:5314252
Synonyms: R26GT
Gene: Gt(ROSA)26Sor  Location: Chr6:113067428-113077244 bp, - strand  Genetic Position: Chr6, 52.73 cM
Germline Transmission:  Earliest citation of germline transmission: J:181830
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
Mutation detailsA targeting vector was created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "MADM GT" cassette. The "MADM GT" cassette used here (GFPN-terminus-intron-tdT3MycATG-less) was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP first exon; nucleotide 1-274), a beta-globin intronic sequence (containing a frt-flanked region of two lox site variants [lox2272 and lox5171] and then a loxP-flanked neomycin resistance gene), an ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire construct was subcloned into the pROSA26-PA targeting vector to generate the final targeting construct pROSA26-GT. The pROSA26-GT construct was inserted into the Gt(ROSA)26Sor locus. (J:181830)
Generation of various Gt(ROSA)26Sor alleles
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  572 strains or lines available
Original:  J:181830 Tasic B, et al., Extensions of MADM (mosaic analysis with double markers) in mice. PLoS One. 2012;7(3):e33332
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory