Igf2^tm4Wrk
Targeted Allele Detail

Summary

• Symbol: Igf2^tm4Wrk
• Name: insulin-like growth factor 2; targeted mutation 4, Wolf Reik
• MGI ID: MGI:5309176
• Synonyms: Rr15^tm4Wrk, S1.2Neo
• Gene: Igf2 Location: Chr7:142204503-142220553 bp, - strand Genetic Position: Chr7, 87.99 cM
• Alliance: Igf2^tm4Wrk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:178292
• Parent Cell Line: CGR8 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutation: Insertion
• Mutation details: A loxP-flanked neomycin resistance cassette has been inserted into the fourth of five HpaII sites within the LRRFIP1/GCF2 (leucine-rich repeat in FLII-interacting protein 1/GC-binding factor 2) repressor protein consensus binding sequence in the first differentially methylated region (DMR1) upstream of the imprinted insulin-like growth factor 2 (Igf2) gene. (J:178292)
• Inheritance: Other (see notes)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igf2 Mutation: 25 strains or lines available

Notes

ES Cell LIne = CGR8.8, derived from 129P2/OlaHsd

This mutation affects the level of gene expression and expression dependent phenotypes in a parent of origin specific manner by interfering with the gene's imprinting.

References

• Original: J:178292 Eden S, et al., An upstream repressor element plays a role in Igf2 imprinting. EMBO J. 2001 Jul 2;20(13):3518-25
• All: 1 reference(s)