Kdm5b^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kdm5b^{tm1c(EUCOMM)Wtsi}
• Name: lysine demethylase 5B; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5308434
• Synonyms: Jarid1b^F
• Gene: Kdm5b Location: Chr1:134487916-134560621 bp, + strand Genetic Position: Chr1, 58.24 cM, cytoband E4
• Alliance: Kdm5b^{tm1c(EUCOMM)Wtsi} page
• IMPC: Kdm5b gene page

Mutation origin

• Mutant Cell Lines: EPD0027_1_F07, EPD0027_1_F08
• Germline Transmission: Earliest citation of germline transmission: J:180267
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 136491938 of Chromosome 1 upstream of the critical exon(s) (Build 37). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 136492740. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. (J:155845, J:173534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kdm5b Mutation: 202 strains or lines available

Notes

Cell lines EPD0027_1_F07 and EPD0027_1_F08 were successfully used to make chimeric mice. Germline transmission was accomplished. J:180267

References

• Original: J:180267 Schmitz SU, et al., Jarid1b targets genes regulating development and is involved in neural differentiation. EMBO J. 2011 Nov 16;30(22):4586-600
• All: 8 reference(s)