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Transgene Detail
Symbol: Tg(ACTB-eGFP,-RAMP1)#Afru
Name: transgene insertion, Andrew F Russo
MGI ID: MGI:5306398
Synonyms: GFP-hRAMP1, Tg(ACTB-eGFP,-RAMP)#Afru
Transgene: Tg(ACTB-eGFP,-RAMP1)#Afru  Location: unknown  
Strain of Origin:  C57BL/6J x SJL/J
Transgene Type:    Transgenic (Inserted expressed sequence)
Mutation:    Insertion
Tg(ACTB-eGFP,-RAMP1)#Afru expresses 1 gene
Mutation detailsThis transgene consists of human RAMP1 cDNA under the control of the CX1 promoter, which is a hybrid of the chicken beta-actin promoter and CMV enhancer. The CX1 vector into which RAMP1 cDNA was inserted also contains an eGFP gene flanked by loxP sites following the promoter (and upstream of RAMP1) and a beta-actin intron and bovine globin polyadenylation signal. CX1 drives ubiquitous expression of GFP, but not RAMP1 due to a translation stop codon and a polyadenylation signal in the GFP cDNA. Not until cre-mediated excision of the GFP-stop sequence at the flanking loxP sites occurs, that RAMP1 is expressed. (J:120077)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Phenotypic Similarity to Human Syndrome: migraine, susceptibility to (J:179493)
Original:  J:120077 Zhang Z, et al., Sensitization of calcitonin gene-related peptide receptors by receptor activity-modifying protein-1 in the trigeminal ganglion. J Neurosci. 2007 Mar 7;27(10):2693-703
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory