Tg(mIGHMdeltaV)1Corc
Transgene Detail

Summary

• Symbol: Tg(mIGHMdeltaV)1Corc
• Name: transgene insertion 1, Daniel Corcos
• MGI ID: MGI:5305993
• Synonyms: delta-V mu m 1, deltaVmum-1
• Transgene: Tg(mIGHMdeltaV)1Corc Location: unknown
• Alliance: Tg(mIGHMdeltaV)1Corc page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2 or (C57BL/6 x SJL/J)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(mIGHMdeltaV)1Corc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  IGHM (3507)

  Ighm	(MGI:96448)

• Mutation details: The deltaVmu_m transgene construct was derived from a rearranged human heavy-chain gene modified (by deletion of the cleavage and polyadenylation signals) so it can produce only the membrane-bound, and not the secreted, mu constant region (Cmu). The V-segment leader exon (L) was joined directly to an 11-kb fragment containing the IGH enhancer and Cmu exons and enhancer, deleting the V(D)J exon. As expected, no human IGmu chains are detected by ELISA in sera from these mice. Transgene-derived mRNA is detected in the spleens of mice of this transgenic line, and spleen cells can be identified by FCM analysis that express both the B cell lineage-specific marker, B220, and the truncated human IGmu chain on their surfaces. (J:135417)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

This was one of three mouse lines derived using this transgene construct and the one characterized in greatest detail. (J:135417)

References

• Original: J:135417 Corcos D, et al., Allelic exclusion in transgenic mice expressing a heavy chain disease-like human mu protein. Eur J Immunol. 1991 Nov;21(11):2711-6
• All: 1 reference(s)