Tg(GFAP-lacZ)9Nist
Transgene Detail

Summary

• Symbol: Tg(GFAP-lacZ)9Nist
• Name: transgene insertion 9, Monica Nister
• MGI ID: MGI:5305741
• Synonyms: GFAP promoter-lacZ, hGFAPprom-LacZ line9
• Transgene: Tg(GFAP-lacZ)9Nist Location: unknown
• Alliance: Tg(GFAP-lacZ)9Nist page

Transgene origin

• Strain of Origin: C57BL/6 x CBA

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: This transgene comprises a 1.8 kb 5' fragment of the human glial fibrillary acidic protein (GFAP) gene driving expression of the E. coli beta-galactosidase (lacZ) coding sequence. X-gal staining of brain sections of developing mouse embryos showed expression to be limited to the central nervous system (CNS), including the eye, and to vertebral chondrocytes forming around the notochord at embryonic day (E) 14.5. Expression occurs in different CNS regions at specific times during development, reflecting the expression pattern of endongenous mouse Gfap mRNA detected by reverse transcription-polymerase chain reaction (RT-PCR) anaylsis and RNA in-situ hybridization. (J:71425)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Transgene expression was analyzed in detail in two transgenic lines, identified as 9 and 99. As no differences were noted, the data are presented without specifying the line used. (J:71425)

References

• Original: J:71425 Andrae J, et al., A 1.8kb GFAP-promoter fragment is active in specific regions of the embryonic CNS. Mech Dev. 2001 Sep;107(1-2):181-5
• All: 1 reference(s)